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As GPCR recycling can be controlled by PKA (Vistein and Puthenveedu, 2013), we also wondered whether our observation of reduced PKA signalling despite similar occupancy for lixisenatide versus exendin-4 (Supplementary Figure 1G) might explain their different recycling rates

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Coelenterazine H (5 M) was then added to the plates and luminescence signals measured 1 h thereafter using an EnVision multilabel plate reader (PerkinElmer) at 18 s intervals before and after ligand addition (25 C)

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