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The practice is based on: (1) established GHRH-ghrelin receptor synergy in endocrine physiology, (2) individual pharmacological data for each peptide, and (3) clinical experience from peptide therapy practitioners

ECG recording ECGs were recorded continuously in deeply anesthetized rats for all three main leads, by positioning stainless steel electrodes on all four limbs using an ECG monitor with a 2090 programmer (Medtronic, United States) connected to a Waverunner LT342 digital oscilloscope (LeCroy, United States)

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Pharmaceutical chemicals with a defined mode of action are included, so that there is sufficient coverage of mechanistically well understood weak-moderate actives, such that target specificity is addressed as well as reasonably possible