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As the NNT mutation decreases the effectiveness of NADPH-related antioxidant pathways, the metabolic impairments produced by the NNT mutation in C57BL6/J mice might be able to be considered a constitutive model of oxidative stress

For these individuals, introducing supplemental sulfur donors like glutathione or N-Acetyl-l-Cysteine (NAC) can sometimes trigger paradoxical mast cell reactions, causing symptom flares, hives, or worsened tachycardia

These may include mild nausea, fatigue, lightheadedness, and flu-like symptoms

RT-qPCR was carried out using Fast SYBR Green Master Mix (Thermo Fisher 4385610) and the mitochondrial/nuclear DNA ratio was derived as described previously 137