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doi: 10.1007/s40519-014-0130-7 22 TakayamaNAmamiyaTNishkawaKYoshizuJAriyoshiHSuzakiYet al

Measurements of energy expenditure and locomotor activity HFD-induced obese C57BL/6J male mice were placed in individual small acryl calorimeter chambers with free access to HFD and water, and respiratory gas (O 2 and CO 2 ) were measured every 5 min for each individual mouse by indirect calorimetry system connected to mass spectrometer (Arco2000, ArcoSystem, Chiba, Japan)

The shot is typically administered in the upper arm, and are considered safe for those with a diagnosed deficiency

Interestingly, mitotically active young muscle has been found to be most responsive to MGF, which argues against a direct effect on quiescent satellite cells [64]