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Basal insulin was measured in plasma from female [Young ( n = 16), Old Con ( n = 26), and Old mAb ( n = 25)] and male mice [Young ( n = 17), Old Con ( n = 32), and Old mAb ( n = 28)] using a bead-based assay for mouse insulin (EMD Millipore, Inc) and detection was performed on a Bio-Plex MAGPIX Multiplex Reader (Biorad Inc., Hercules, CA)
Arabidopsis thaliana mitochondrial glyoxalase 21 exhibits beta-lactamase activity
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Digest solution: RPMI (DMEM or IMDM) containing 5% fetal calf serum (FCS), 10 U/ml collagenase (see Note 1)
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