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Nevertheless, subsequent deprotonation of the analyte by counterion neutralization is thermodynamically unfavorable because the acidity of the carboxylic acid groups of peptides and proteins is lower than that of common counterions (such as trifluoroacetic acid (TFA) or chloride)
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Dot blot Commercially available human recombinant pro-BDNF (Alomone Labs) and two types of mature-BDNF (Sigma and Alomone Labs) were spotted on a nitrocellulose membrane at the concentration of 10 pg and 1000 pg per lane, respectively
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