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Upon request, a topical numbing agent can be purchased and applied, to minimize any discomfort

Immunoblot assays Tissue samples were crushed on dry ice to obtain homogenous aliquots and then lysed in RIPA buffer (Thermo Scientific #89900) containing Halt protease & phosphatase inhibitor (Thermo Scientific #1861280)

Int J Nanomed 18:60776099 Ahmad MY, Liu S, Tegafaw T, Saidi AKAA, Zhao D, Liu Y et al (2023) Heavy metal-based nanoparticles as high-performance x-ray computed tomography contrast agents

Gastrointestinal: Abdominal pain, nausea, vomiting, diarrhea, constipation, bloating, and gastroesophageal reflux frequently misdiagnosed as functional GI disorders