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Furthermore, alogliptin prevented the phosphorylation and degradation of IB, nuclear translocation of NF-B p65 and suppressed IB/NF-B signaling [35]

After drying of peptides in a vacuum concentrator, peptides were desalted by solid phase extraction (Oasis HLB 96-well Elution plate, Waters) according to the manufacturers instructions and ~100 ng per sample was used for mass spectrometric analysis

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