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Specifically, we dissect how baseline microbial signatures correlate with differential efficacy, while critically assessing clinical confounding factors, such as metformin co-treatment and dietary shifts, and the methodological limitations that currently hinder the translation of microbiome profiling into precision endocrinology

For measurements with DTT, GSH, and TCEP, a fivefold excess of these reagents was used relative to the cysteine residues

In addition, unsupervised principal component analysis (PCA) showed partial separation between MMA and healthy control samples, with some overlap