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Exendin-4 shares approximately 53% structural similarity with human GLP-1 but is resistant to DPP-4 enzyme degradation, giving it a significantly longer half-life than native human GLP-1, which breaks down in approximately 2 minutes

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Increased plasma xanthine and hypoxanthine can also occur consequent to disorders of mitochondrial oxidative phosphorylation or of glycogen metabolism (77)

Adding protease inhibitors such as 1mM PMSF to cell lysates does not inhibit binding or elution of polyhistidine-tagged proteins with the HisLink Resin and is highly recommended to prevent degradation of the protein of interest by endogenous proteases