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Subsequent work established that the same sequence exists in human plasma, saliva, and urine at concentrations that decline with age, and that the sequence is found as a subfragment of the alpha-2 chain of type I collagen, which means GHK is released into the tissue environment when collagen breaks down

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To test the involvement of NRF2, we bred the Gclc f/f mouse strain with the Nrf2 f/f mouse strain 57,58 and induced a liver-specific double deletion with the AAV-TBG-Cre virus (hereafter referred to as L-DKO) (Fig