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Signal was amplified with biotinylated secondary antibody and horseradish peroxidase (HRP)-conjugated streptavidin and visualized with 3,3-diaminobenzidine (DAB) (Solarbio, cat

The company has received Fast Track and Breakthrough Therapy designations from the FDA for pemvidutide in the treatment of MASH and AUD, which may expedite the development and approval process for this promising therapy

In brief, chemical standard libraries of 149 metabolites in seven pooled libraries were prepared and serially diluted in high-performance liquid chromatography (HPLC)-grade water from in a dilution series from 5 mM to 1 M to generate external standard pools, which are used for calibration of isotopically labelled internal standards and to quantify concentrations of metabolites for which internal standards were not available