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KEAP1 mass spectrometry analysis For mass spectrometry analysis, 10 g of recombinant human KEAP1 (#11981-H29B-100, Sino Biologicals, Beijin, PR China) were diluted to 100 l with 20 mM Tris pH 8.0 and diafiltrated by means of a Amicon Ultra-0.5 ml device (Sigma-Aldrich, Milan, Italy) to eliminate GSH, and then treated with 30 mM M DTT and incubated 30 min at room temperature to reduce the disulfide bridges, diafiltrated to remove the excess of DTT and diluted in 20 mM Tris pH 8.0

Whether the topical form can effectively penetrate the skin barrier and be absorbed by the muscles remains unclear

Carefully manage the use of diuretics and RAAS blockers (e.g., ACE inhibitors, ARBs)

Sturtz, L.A., Diekert, K., Jensen, L.T., Lill, R