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Six to eight-week-old female SD rats (about 160 g) were anesthetized

Cell culture The human melanoma cell lines SK-MEL-147 (Memorial Sloan Kettering Cancer Center) and WM88 (Rockland, Cat #WM88-01-0001), the glioblastoma cell line ONDA7 (JCBR, Cat #JCBR1575), the breast cancer cell line SK-BR-3 (ATCC, Cat #HTB-30), the human kidney cell line HEK293T (ATCC, Cat #CRL-3216), and the mouse melanoma cell line B16F10 (ATCC, Cat #CRL-6475) were cultured in high glucose DMEM (Gibco), fortified with 10% syringe-filtered FBS (Gibco), 1% penicillin/streptomycin (Gibco), and 0.2% Normocin (InvivoGen)

Step 6: Inject bateriostatic water into the Melanotan-2 vial, slowly and ensure the water does not come in direct contact with the powder, instead inject so that the water falls onto the side of the vial and hits the glass before it comes into contact with the powder - Melanotan-2 alongside all peptides are very sensitive compounds

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