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RNAscope Tissue collection Glp1r OBhM3Dq mice ( n = 3, previously injected with viruses expressing hM3Dq-mCherry under the Glp-1r promoter control) were anaesthetised with sodium pentobarbital (50 mg/kg saline) and transcardially perfused with PBS 1X followed by 4 C PFA 4% for 5 min

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