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Chugais medicinal-chemistry program ran multiple cycles of structureactivity optimization to arrive at OWL833/LY3502970, tuning both receptor affinity and the drug-like properties (solubility, permeability, metabolic stability) that a peptide simply cannot achieve

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The supernatant was then adjusted to pH 4.0 with acetic acid, loaded onto a HiTrap Q HP column that was installed on FPLC KTA purifier system (GE Healthcare) and pre-equilibrated in pH 4.0 water, and eluted with a linear gradient of pH 4.0 water supplemented with 500 mM NaCl

Laboratory testing may also be recommended if glandular function, insulin sensitivity, or hormone balance are involved