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The peptide's actin-sequestering activity proves particularly important for endothelial cell migration, with studies showing TB-500 significantly enhances endothelial cell migration rates and tube formation in angiogenesis assays while promoting the formation of stable, mature blood vessels with appropriate pericyte coverage

Aliquots of cell pellets and concentrated culture media were either used for GFP fluorescence measurement or stored at 20 C for Western blot analysis

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