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Since the number of sites is limited, as more sites are occupied by GSH Px from the sample, fewer sites are left to bind GSH Px-HRP conjugate

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This 1:1 ratio prevents peptide precipitation (which occurs in overly concentrated solutions) while avoiding excessive dilution that wastes refrigerator space and increases contamination risk across multiple draws

As a result, the BW25113 strain and its caiA mutant have become vital to our maintenance of gbh-1 mutants and subsequent assessments of -BB toxicity and metabolic studies, as the WT strain supports growth and embryonic viability, and the caiA mutant strain supports growth (for one generation), yet does not support embryonic viability