This work was financially supported by the Yunnan Clinical Research Center for Rheumatism in Traditional Chinese Medicine (202405AJ310004), the Yunnan Science and Technology Talent and Platform Program (202105AG070012), the Yunnan University of Chinese MedicineHospital Joint Fund Projects (XYLH2024042 and XYLH2024030), the Science Research Foundation of Education Department of Yunnan Province (2025Y0638 and 2024Y380), the Expert Workstation of Zhangxuan in Yunnan Province (202305AF150175), the Funding of Yunnan Applied Basic Research Projects-Union Foundation (202401AZ070001-052) and the High level Key Discipline of TCM Construction Project, Rheumatology, of National Administration of Traditional Chinese Medicine (zyyzdxk-2023189)
Skipping Cycling Protocols The Problem: Continuous daily use without planned off-periods, assuming "more is better." Why It Matters: Prolonged use without breaks may lead to receptor desensitization, reduced effectiveness, and potential unknown long-term effects
The main outcomes would be cardiovascular and cerebrovascular events, and the prognosis could be improved through aggressive intervention against metabolic abnormalities
For each treatment, one column contained 30g/ml of digitonin as a positive control for cytotoxicity
