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Pericytes themselves help maintain BBB integrity and decrease paracellular permeability of endothelial cells by enhancing the formation of tight junctions and maintaining low levels of transcytosis in the brain endothelium [35,36,37]
Although we cannot rule out that the increased production of ROS in 3D culture conditions may not be observed under hypoxia, it should be noted that hypoxia itself induces ROS production in numerous cell types, including tumor cells [39]
Other data that support the findings of this study are available upon request
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