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After washing with PBS (3 5 min), sections were incubated with species-matched Alexa Fluor-conjugated secondary antibodies (1:500, Invitrogen) for 2 h at RT protected from light

f , Immunoblot analysis of indicated proteins in whole-cell lysates and mitochondria isolated from SLC25A39 knockout HEK-293T cells expressing a vector control, mito-GshF or SLC25A39 cDNA

Muscle fills some of the volume that fat previously occupied, creating a more taut surface for the skin to drape over

Research and clinical experience suggest that IV NAD+ produces a sharp but short-lived spike in plasma levels that does not reliably translate to intracellular increases, which is where the therapeutic effect actually happens