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In vivo, aged mice administered ASHMI (9.5 mg/ mouse per day) saw similar results to young mice, including significantly decreased lung inflammation, airway mucous metaplasia, downregulated MUC5AC [100]

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Anxiety, depression, and chronic stress amplify pain signals and affect recovery

Mechanisms of action Preclinical studies document: Anxiolytic effect without sedation in classical tests (elevated plus maze, open field, light-dark box) GABAergic system modulation : allosteric potentiation of GABA-A observed ex vivo Enkephalin increase (endogenous opioid peptide) and serotonergic modulation Immunomodulatory effects inherited from tuftsin: macrophage activation, T-helper modulation Behavioural models Anxiolysis is measured via time spent in open arms of the elevated plus maze (EPM), visit ratio of central zones of open field , and exit latency in light-dark box