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2014 May;3(3):1624

Clinical studies have found that many patients lose a meaningful percentage of their body weight over one to two years, especially when medication is combined with healthier eating habits and increased physical activity

Briefly, adult fat bodies were dissected in 1 phosphate buffered saline (PBS), fixed 20 min in 4% paraformaldehyde at room temperature (RT), rinsed 5 in 1 PBS, then permeabilized for 30 min at RT in 1xPBS + 0.3% Triton X-100

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