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The powdered tissue was lysed in RIPA lysis buffer (Servicebio, G2002) supplemented with 1% phosphatase inhibitor (Epizyme, GRF102) and 1% protease inhibitor (Epizyme, GRF101), followed by agitation at 4 for 30 min

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By stimulating osteoblast activity (the cells responsible for building bone), certain peptides can promote: Fracture healing Post-surgical bone recovery Improved bone density in conditions like osteoporosis This opens up exciting possibilities for patients dealing with slow-healing fractures or age-related bone loss

L-Theanine is a naturally occurring amino acid found primarily in green tea