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Total RNA of HBE cells and lung tissues were extracted using a GenElute TM Mammalian Total RNA Miniprep Kit (Sigma, U.S.) according to the manufacturers protocol, and the concentration of total RNA was determined by measuring the absorbance at 260 nm using a Nanodrop 2000c spectrophotometer (Thermo Scientific, U.S.)

Vitamin B12 Deficiency Your body stores 1,000-2,000 times as much vitamin B12 as you'd eat in a day, so it can take many years to see symptoms of vitamin B12 deficiency