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The cells were then fixed with frozen methanol for 15 min at 4 C and washed with PBS three times, followed by the addition of DAPI (4,6-diamidino-2-phenylindole, Beyotime Biotechnology Co

Cambridge: Woodhead Publishing

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Pinch a layer of skin with your thumb and index finger, which should be about an inch and a half apart from each other, in the front area of the thigh