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SNAP-FLAG-hGLP-1R was labelled for 15 minutes at 37 C with SNAP-Surface Alexa Fluor 546 or 647 in full media prior to stimulation with 100 nM exendin-4 and imaging in a Leica Stellaris 8 inverted confocal microscope with a 63/1.40 NA oil immersion objective with Lightning confocal super resolution modality from the facility for imaging by light microscopy (FILM) at Imperial College London

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General safety considerations relevant to injectable medicines in a clinical trial context include: All needles and syringes used in preparation or administration must be disposed of in an approved sharps container

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