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In an additional embodiment the formulation or composition provides a therapeutically effective amount of at least one otic agent at the site of disease with essentially no detectable systemic exposure

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For ER36 expression, 1 10 6 cultured breast cancer cells or cells from xenograft tumors were incubated with 10 L of anti-ER36 antibody at 4 C for 40 min, followed by incubation with 0.5 L goat anti-mouse Alexa Fluor 647 antibody (Sparks, MD) at 4 C for 30 min