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Materials and methods Cell culture and treatments Human 21NT breast cancer cells were cultured in 1 Modified Eagles medium alpha (alpha MEM) (Gibco), 2.8 M hydrocortisone, 1 g/ml insulin, 10% foetal calf serum (FCS), 1% gluta- max, 10 mM HEPES, 0.1 mM NEAA and 12.5 ng/ml Epithelial Growth Factor (EGF)

doi: 10.1038/s41467-022-35199-0 47

N-acetylcysteine administration Intravenous N-acetylcysteine is the antidote to treat paracetamol overdose and is virtually 100% effective in preventing liver damage when given within 8 hours of the overdose

The system reported an overall gain of $1.2 billion in 2023, including a nonoperating gain of $1.1 billion