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Methods We performed a combined retrospective and prospective single centre, cohort study of patients who received semaglutide to assist in weight loss at a single bariatric centre

Upon arrival, islets were resuspended in serum media (RPMI-1640 + 10% FBS + 5 mM glucose [for a final glucose concentration of 16.1 mM] + 1% antibiotic-antimycotic) and allowed to recuperate in the incubator set at 37C and 5.0% CO 2

This reflects the body composition shift rather than weight loss in the conventional sense

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