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In organotypic hippocampal slice cultures, Dihexa and Nle-AngIV similarly augmented spinogenesis, with control slices showing 7 spines per 50-m dendrite length, compared to 11 for both Dihexa and Nle-AngIV treated slices [1]
They were allowed 60 seconds to search the tank for the platform and at the end of each session they were placed on the platform and remained there for 30 seconds
Culture media from 24-hour incubated cells were collected, stored at -80 C, and analyzed for urea concentration using LC/MS/MS API3000, normalized to total cell protein
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