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The assessment of neuronal activation using cFos as a surrogate marker in response to an acute administration of cagrilintide and sCT in chow-fed mice revealed that both cagrilintide and sCT produced a significantly higher cFos signal compared to vehicle-treated mice, showing similar activation patterns in WT mice AP, NTS and LPBN brain areas

The bedtime timing matters because the goal is to amplify a pulse that would otherwise occur naturally during deep sleep, not to introduce a daytime pulse that conflicts with normal pituitary rhythms