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4.5 Live cell imaging Confocal images of 4 optical planes interspaced by 1 m (from -1.0 m to 2.0 m included) across 25 x 25 fields of view with a 10% overlap were acquired per well on an Opera Phenix High Content Screening System (PerkinElmer) using a 40x or 63x/1.15 NA water-immersion objective, with live cell parameters (37 C and 5% CO 2 )

This 5-year follow-up to the STAMPEDE clinical trial randomized patients with T2DM to RYGB, VSG or medical management, and showed that RYGB and VSG were superior to medical therapy in terms of weight loss, glycaemic control and reduction in medication use

GLP-1 receptor agonists like semaglutide are peptides that influence appetite, blood sugar, and metabolism

As herbs are known for the multiple target characteristics, several hormones could be controlled at the same time, and the increase or decrease levels of hormone also could vary for each experiment