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Samples were thawed at room temperature and cytokine concentrations (IL-2, IL-4, IL-6, IL-10, IL-17A, IFN, TNF, soluble Fas, soluble FasL, granzyme A, granzyme B, perforin and granulysin) was quantified using the LEGENDplex Human CD8/NK V02 Panel (BioLegend, 741187), according to the manufacturers instructions

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It has been one of the most extensively studied copper peptides in dermatological and wound biology research, with over 100 published studies examining its effects on extracellular matrix remodeling, collagen synthesis, and tissue signaling