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The cells were then cultured for an additional 48 h, followed by maintenance in high-glucose DMEM medium supplemented with 10% FBS

Gonadotropin-releasing hormone (GnRH) drives luteinizing hormone (LH) and follicle-stimulating hormone (FSH) release

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Unfortunately, VEGF can also be detrimental because its overexpression can cause inflammation in the nervous system, which in turn can lead to BBB leakage, promote the production of pro-inflammatory cytokines and the occurrence of neurodegeneration (Claire and Larrive 2017