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After treatments, the slides were fixed with 4% paraformaldehyde at 37C for 30 min, permeabilized with 0.2% Triton X-100 (Beyotime Institute of Biotechnology) at 37C for 20 min, blocked with 5% bovine serum albumin (BSA, Beijing Solarbio Science & Technology Co., Ltd.) at 37C for 30 min and incubated with primary antibodies against HMGB1 (1:100) and GPX4 (1:100) at 4C overnight

Specifications Research-grade material supplied in lyophilized vial presentation at 10mg/10mg

Lactobacillus gasseri BNR17 and Limosilactobacillus fermentum ABF21069 ameliorate high sucrose-induced obesity and fatty liver via exopolysaccharide production and -oxidation