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G s conformational change HEK293A cells stably expressing the GLP-1R (tested and confirmed to be free from mycoplasma) were transfected with a 1:1:1 ratio of G 2 :venusG s :nanolucG 1 using a standard PEI protocol (6:1 ratio PEI:DNA)

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Lymphoproliferative response Cats offered the diet supplemented with 15 % free GLY had ConA-induced lymphoproliferative activities not significantly different to control group cats throughout the test phase (all P 035) (Table 4)