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In vertebrates, photoreceptors are some of the cells with most active metabolism, and even compared to the brain, they use more glucose as a metabolic fuel 8

doi: 10.1016/j.physbeh.2004.01.007

The resulting pellet was re-suspended in minimal 50 mM HEPES pH 8.0, 200 mM NaCl, applied to a 1545% w/v sucrose gradient and subjected to ultracentrifugation at 4 C for 3 h, 105,000 g

When required, erythromycin (10 g/mL for S