Membranes were solubilized in resuspension buffer (50 mM Tris-Cl pH 7.0, 300 mM NaCl, 0.5% 2,2-didecylpropane-1,3-bis--D-maltopyranoside (LMNG)/0.05% cholesteryl hemisuccinate (CHS) supplemented with protease inhibitor as described above) at a ratio of 15 mL/g of membrane at 4 C for 4 h
These pharmacokinetic differences inform the trade-offs in onset, convenience, and dosing convenience
Effect of low pH on glutamate uptake and release in isolated presynaptic endings from rat brain
On entering the body, SS-31 binds with high affinity to cardiolipin, a phospholipid uniquely found in the inner mitochondrial membrane and known to interact with a protein called cytochrome c.2 This interaction determines whether cytochrome c becomes either an electron carrier or a peroxidase, and SS-31 promotes conversion to the former instead of the latter.3 As an electron carrier, cytochrome c drives the synthesis of adenosine triphosphate (ATP), our primary form of cellular energy.4 ATP production is the principal function of mitochondria, so SS-31s presence helps ensure that the powerhouses of the cell continue to carry out their critical role
