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Pharmacological management of nutrient-related metabolic disturbances is crucial, as evidenced by the effectiveness of SGLT2 inhibitors such as canagliflozin in lowering the risk of kidney failure and cardiovascular events in patients with type 2 diabetes and established nephropathy (117, 118)

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Islets were then dispersed into single cells with calcium-free Hanks solution and used after incubation for another 24 hours in RPMI media containing either 5.6 mM glucose or 25 mM glucose

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