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HepG2 cells were grown on cover slips and treated with ASA after GSH depletion with or without NAC as described in the Materials and Methods

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It replenishes glutathione levels, helps repair oxidative damage, and supports recovery from toxin exposure or alcohol-induced stress

We showed that mice fed a high-fat diet (HFD) for 12 weeks developed hepatic steatosis and liver injuries, which were associated with not only TNF overproduction but also hepatic glutathione dysregulation, characterized by GSH reduction and GSSG elevation