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[21] Within cells, cyanocobalamin is converted to methylcobalamin and 5- deoxyadenosylcobalamin, coenzymes that participate respectively in homocysteine remethylation to methionine and in the isomerization of methylmalonyl-CoA to succinyl CoA, reactions essential for DNA synthesis, erythropoiesis, and myelin maintenance

10 L of diluted plasma is added to 15 mL centrifuge tubes and digested in the presence of 362 L hydrogen peroxide (H 2 O 2 ) and 1,086 L of 67%69% concentrated nitric acid (HNO 3 ) for 90 min in water bath at 60 C

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