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Description

The reducing capacity of GSH is essential for regulating the redox environment, as it can reversibly form oxidized glutathione (GSSG) through the coupling of two GSH molecules

To quantify total PDHc-E2 and OGDHc-E2, lipoate blots were stripped using Restore Western Blot Stripping Buffer (Thermo Fisher Scientific, 21059), reblocked in 5% skim milk in PBST and re-immunoblotted using appropriate primary antibodies