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The cells were maintained in 5% CO2 at 37C in RPMI-1640 medium (Thermo Fisher Scientific), supplemented with 10% fetal bovine serum (Thermo Fisher Scientific), 1% HEPES (Gibco), 1% Pen-Strep (Gibco), and 2.2% glucose (Gibco)

It is necessary to thoroughly understand the basis of metal-based NP toxicity before extensive metal-based NPs can be safely applied to human beings

People with certain FTO and MC4R gene variants often experience more pronounced appetite resurge after GLP-1 discontinuation, which is why personalized strategies matter more than one-size-fits-all advice

While these medications are similar, they have some key differences