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Immunohistochemical staining by an automated immunostainer (Dako Autostainer Plus, DakoCytomation, Glostrup, Denmark) was performed using LSAB HRP and HRP+ kits according to the manufacturers instructions
Suboptimal medication use comprises (1) underprescribing, (2) polypharmacy or overuse, and (3) high-risk prescribing, such as potentially inappropriate medications (PIMs), prescribing cascades, and anticholinergic and sedative effects
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