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Multi-omics approaches, such as metabolomics to identify lipid peroxidation profiles and genomics to characterize polymorphisms in ferroptosis-regulating genes, may allow more accurate pathological classification

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Lysates were combined with 6 Laemmli SDS sample buffer (Boston BioProducts, BP-111R) containing 5% 2-mercaptoethanol (VWR Life Science, M131), then loaded onto 420% Criterion TGX pre-cast gels (Bio-Rad, 5671093)