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Total phenolic substance A 0.5 mL aliquot of the extracted walnut powder sample was transferred to a reaction vessel, followed by the sequential addition of 2 mL of 0.2 N FolinCiocalteu reagent and 2.5 mL of sodium carbonate (NaCO) solution

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[4] These interactions support the study of enzyme kinetics, substrate specificity, thiol-disulfide exchange, and protein modification under controlled laboratory conditions

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