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In the reduced state, HMGB1 acts as a chemoattractant

The histologic classification of 602 cases of feline lymphoproliferative disease using the National Cancer Institute working formulation

doi: 10.3389/fmed.2021.689042 Received 31 March 2021 Accepted 15 June 2021 Published 09 August 2021 Volume 8 - 2021 Edited by Oscar Escribano, Complutense University of Madrid, Spain Reviewed by Adriaan Holleboom, Academic Medical Center, Netherlands

The remaining fat particles were digested with 0.2% collagenase (Gibco, Billings, MT, USA) in a water bath shaker at 37 C for 30 min, and the mixture was centrifuged at 1000 rpm for 5 min to remove undigested fat