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Confirming the Diagnosis MRI scan helps diagnose a TFCC tear

In addition, we transfected BRAF v600E /shPTEN transformed melanocytes with siEPAC1 or siEPAC2 and/or siEPAC1/2

Before use, the frozen hind paws were thawed and cut into small pieces and resuspended in 1 ml phosphate buffered saline (PBS, pH 7.4) containing a 1x complete protease inhibitor cocktail tablet (Roche Diagonostic GmbH, Mannheim, Germany) and homogenized by shaking with 1.5-mm glass beads in a Mini-BeadBeater (BioSpec, Bartlesville, OK, USA) at 4,800 oscillations/min for 3 min