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The powdered tissue was lysed in RIPA lysis buffer (Servicebio, G2002) supplemented with 1% phosphatase inhibitor (Epizyme, GRF102) and 1% protease inhibitor (Epizyme, GRF101), followed by agitation at 4 for 30 min

This approach has been adopted by Novo Nordisk to create one of the first oral formulations of a long, linear peptide drug (semaglutide), which is widely adopted on the market under the brand name Rybelsus 5

[27] Amberger JS, Hamosh A

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